A pond is considered as an ecosystem with a boundary drawn around the edge of the water. What makes this pond an open system?
Matter can cross the boundary but energy remains within it.
Neither energy nor matter can cross the boundary of the pond.
Both energy and matter can cross the boundary of the pond.
Energy can cross the boundary but matter cannot cross it.
Saprotrophic fungi grow through fallen leaves on a forest floor. What is their immediate supply of energy?
Heat released from the soil during decomposition
Chemical energy in carbon compounds in dead organic matter
Inorganic ions absorbed from the surrounding soil water
Light absorbed directly by fungal hyphae
In the food chain phytoplankton copepod herring seal, what is the trophic level of the herring?
Primary consumer
Secondary consumer
Producer
Tertiary consumer
What description best defines a heterotroph in this topic?
An organism that uses carbon dioxide and light to synthesize all mineral ions
An organism that obtains carbon compounds from other organisms and assimilates them into its own tissues
An organism that recycles heat energy from the environment into food-chain biomass
An organism that releases oxygen by oxidizing carbon compounds in cell respiration
A student constructs a quantitative energy pyramid for a grassland food chain. What feature is required for the pyramid to represent energy transfer correctly?
Bars show energy content per gram only, with decomposers as the top level.
Bars show carbon dioxide released per unit mass, with equal widths for all levels.
Bars show the number of species present, with carnivores at the base.
Bars show energy available per unit area per unit time, with producers at the base.
A mangrove forest accumulates woody biomass and soil carbon over several decades. Photosynthesis in the ecosystem exceeds respiration and decomposition. How should the ecosystem be classified over this period?
A carbon sink, because there is a net uptake of carbon dioxide
A closed carbon system, because carbon remains in the forest boundary
A trophic level, because biomass increases in producers
A carbon source, because all forests release oxygen during photosynthesis
A small pond is treated as an ecosystem with a boundary drawn around the edge of the water.

Distinguish between an open system and a closed system, using the pond as an example.
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A food chain in a grassland is shown.
grass grasshopper frog snake
Outline what the arrows represent in this food chain.
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Iron-oxidizing bacteria live in acidic mine drainage where light is unavailable. What combination allows these bacteria to be autotrophs?
Energy from heat and carbon from iron(III) ions
Energy from oxidation of iron(II) ions and carbon from carbon dioxide
Energy from light and carbon from dissolved organic sugars
Energy from digestion and carbon from other bacterial cells
The graph shows atmospheric carbon dioxide concentration recorded at a remote monitoring station over several decades. What explains the long-term trend shown by the graph?

Seasonal photosynthesis removes more carbon dioxide each year than combustion releases.
Combustion of fossil fuels adds carbon dioxide faster than natural sinks remove it.
Respiration stops during the growing season and restarts in winter.
Remote monitoring stations produce carbon dioxide by sampling the atmosphere.
In a wetland, gross primary production is and respiration by autotrophs is . What is the net primary production?
A terrestrial ecosystem has abundant plant biomass but supports only a very small population of apex predators. What is the main reason that additional trophic levels are restricted?
Less total energy remains available after losses at each trophic transfer.
Producers stop respiring when consumers feed on them.
Energy is recycled by decomposers before it reaches predators.
Carnivore tissue contains no chemical energy per unit mass.
The diet of a marsh bird changes during the year. In spring it feeds mainly on seeds of reeds. In summer it feeds on herbivorous insect larvae. In late summer it may also feed on small fish that have eaten the larvae.
Classify the marsh bird into trophic levels for the three feeding relationships described.
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Fallen leaves, dead insects and faeces accumulate on the floor of a woodland.
Explain how decomposers obtain energy from this material.
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Iron-oxidizing bacteria are found in acidic drainage from abandoned mines where there is little light but a high concentration of iron(II) ions.
Explain how these bacteria can act as producers in this environment.
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Students investigated a small stream ecosystem by defining a boundary around a 20 m section of stream. They recorded transfers of energy and matter across the boundary during 24 hours.

Identify one transfer of energy and one transfer of matter into the stream section.
State whether the stream section is an open or closed system, using evidence from the diagram.
Suggest why a natural ecosystem can continue to recycle matter but still requires a continuous input of energy.
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A food web was constructed from observations in a meadow community. Arrows show the direction of transfer of energy and biomass.

Identify one producer and one secondary consumer in the food web.
State what the arrows in the food web represent.
Explain why the mouse cannot be assigned to only one trophic level in this food web.
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Dry plant biomass is burned under a calorimeter. The heat released raises the temperature of of water by . The specific heat capacity of water is and the mass of biomass burned is . What is the calculated energy content of the biomass?
The diagram represents part of a carbon cycle. Arrow X goes from atmospheric carbon dioxide to producer biomass. Arrow Y goes from consumer biomass to atmospheric carbon dioxide. What labels should be used for X and Y?

X: photosynthesis; Y: respiration
X: respiration; Y: feeding
X: decomposition; Y: carbon fixation
X: combustion; Y: photosynthesis
Energy available to each trophic level was estimated in a freshwater food chain.

State the trophic level with the greatest energy available.
Explain two causes of the reduction in energy available at successive trophic levels.
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Two organisms are found in the same lake. A green alga grows near the surface. A fish feeds on small crustaceans.
Compare how the alga and the fish obtain and use carbon compounds.
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A student estimated the energy content of dry seeds by burning them beneath a tube containing water. A sample of seed with mass heated of water from to . The specific heat capacity of water is .

Calculate the estimated energy content of the seed sample in .
Suggest one reason why this method may underestimate the true energy content of the seeds.
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The graph shows atmospheric carbon dioxide concentration measured at a remote monitoring station over several decades.

Identify the two main patterns shown by the graph.
Explain the biological and human causes of these patterns.
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A terrestrial ecosystem contains producer biomass, consumer biomass, dead organic matter and atmospheric carbon dioxide.
Construct a labelled carbon cycle diagram for this ecosystem, including photosynthesis, feeding and respiration.
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Light intensity and net photosynthetic rate were measured at different depths in two lakes. Lake A is clear and Lake B is turbid due to suspended particles.
| Depth / m | Light intensity A / % | Light intensity B / % | Net photosynthetic rate A / arbitrary units | Net photosynthetic rate B / arbitrary units |
|---|---|---|---|---|
| 0 | 100 | 100 | 15 | 15 |
| 5 | 85 | 60 | 14 | 11 |
| 10 | 70 | 30 | 13 | 7 |
| 15 | 55 | 12 | 11 | 3 |
| 20 | 40 | 4 | 8 | 1 |
| 25 | 25 | 0 | 5 | 0 |
| 30 | 10 | 0 | 2 | 0 |
Compare the depth of the light zone in Lake A and Lake B.
Explain why the turbid lake has lower net photosynthetic rate at depth.
Suggest one way in which an ecosystem below the light zone could obtain energy.
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Researchers placed samples of leaf litter and animal faeces in mesh bags on a forest floor. Changes in dry mass and fungal hyphal length were recorded over several weeks.

Describe the change in dry mass of the organic material during the investigation.
Explain why faeces can supply energy to decomposers.
Suggest why decomposers are important in the recycling of matter in this forest ecosystem.
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Energy flow was estimated for four trophic levels in a temperate grassland food chain.
| Trophic level | Energy / kJ m^-2 yr^-1 |
|---|---|
| Producers | 10000 |
| Primary consumers | 1200 |
| Secondary consumers | 150 |
| Tertiary consumers | 18 |
Describe the pattern in energy availability shown in the table.
Calculate the percentage transfer efficiency from producers to primary consumers.
Explain two causes of the reduction in energy available to the next trophic level.
Suggest why this ecosystem is unlikely to support a fifth trophic level.
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Iron-oxidizing bacteria were sampled from an acidic stream draining an old mine. The bacteria obtain energy by oxidizing iron(II) ions to iron(III) ions and use this energy to fix carbon dioxide.

Identify the external energy source used by the bacteria in the investigation.
Explain why these bacteria are classified as chemoautotrophs rather than photoautotrophs.
Suggest why similar chemoautotrophs can support ecosystems below the level of light penetration in oceans.
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Measurements were made in a young forest during two consecutive years. In the first year, carbon dioxide uptake by photosynthesis was greater than carbon dioxide release by respiration and decomposition. In the second year, a dry summer was followed by a fire that burned much of the above-ground biomass.
Evaluate whether the forest acted as a carbon sink or a carbon source in each year.
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A sealed terrarium receives light through glass but no matter is added after it is closed. Plants, small animals, fungi, bacteria, soil, air and water are present inside.

Explain why matter can be recycled in the terrarium but energy cannot be recycled.
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Students estimated the energy content of dried seeds using simple combustion calorimetry. A known mass of dry seed was burned below a tube containing water. The temperature change of the water was recorded.

Calculate the energy content of the seed using the data provided.
Explain why the value obtained is likely to be lower than the true energy content of the seed.
State why energy released from biomass combustion cannot be biologically recycled into a food chain.
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Researchers compared carbon production in a young mangrove forest over one year. Production values are expressed as .
| Carbon flux | Annual value / g C m^-2 yr^-1 |
|---|---|
| Gross primary production | 2400 |
| Autotroph respiration | 1120 |
| Secondary production | 640 |
Calculate the net primary production from the gross primary production and autotroph respiration values.
Distinguish between primary production and secondary production using the data.
Explain why secondary production is lower than net primary production in this ecosystem.
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A simplified carbon cycle was prepared for a wetland ecosystem. Carbon pools are shown as boxes and carbon fluxes as arrows.

Identify the process represented by the arrow from atmospheric to producer biomass.
Explain how carbon in consumer biomass can return to atmospheric in this wetland.
Using the diagram, suggest why this wetland may act as a carbon sink over long time periods.
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The graph compares estimated carbon dioxide emissions from combustion of different carbon stores in one region over 150 years.

Identify the carbon store that contributes most to emissions at the end of the period shown.
Explain why combustion of fossil fuels has a different effect on the active carbon cycle from combustion of recently produced biomass.
Suggest why peat fires can cause large net releases of even if the burned area is small.
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A freshwater pond is studied as an ecosystem. Rainwater, dissolved mineral ions, sunlight, migrating insects and fallen leaves can cross the boundary drawn around the pond.
State why the pond is an open system.
Distinguish between an open ecosystem and a closed system.
Explain why a continuous input of energy is needed in ecosystems, whereas matter can be recycled.
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The figure shows feeding relationships recorded in a rocky shore community. Limpets graze on green algae. Small crabs feed on green algae and amphipods. Amphipods feed on detritus and green algae. Fish feed on amphipods and small crabs. Herons feed on fish and small crabs.

Identify the producer in the food web.
Explain why small crabs can be placed in more than one trophic level in this food web.
Discuss the value and limitations of this food web as a model of energy transfer in the community.
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A fallen tree in a humid forest is colonized by fungi, bacteria, woodlice and insect larvae. After several months, the mass of visible dead wood decreases and mineral ions increase in the surrounding soil.
Compare saprotrophs and detritus feeders in how they obtain organic matter from the fallen tree.
State the form in which energy is supplied to decomposers.
Explain the roles of decomposers in both energy transfer and recycling of matter in the forest ecosystem.
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Atmospheric carbon dioxide concentration was measured at a remote monitoring station for several decades.

Describe the two main patterns shown in the graph.
Explain the annual fluctuation in atmospheric concentration.
Suggest why the long-term increase continues even though decreases during part of each year.
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Scientists measured annual global fluxes of carbon dioxide and oxygen associated with photosynthesis and aerobic respiration. Values are shown as relative fluxes.

State the source of atmospheric oxygen used in aerobic respiration.
Explain the mutual dependence between photosynthesis and aerobic respiration shown in the diagram.
Evaluate the claim that the large annual photosynthesis and respiration fluxes prevent combustion from increasing atmospheric concentration.
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Researchers estimated energy available at successive trophic levels in a grassland food chain. The data are shown in an energy pyramid.

Explain why the pyramid should be drawn with the producers at the base.
Using the data, calculate the percentage transfer efficiency from producers to primary consumers.
Evaluate the statement that the pyramid narrows because each gram of carnivore tissue contains less energy than each gram of producer tissue.
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A wetland restoration project measured carbon fluxes before and after re-flooding. The measurements included gross primary production, respiration by organisms and accumulation of dead plant material.
| Condition | GPP / g C m^-2 yr^-1 | Respiration / g C m^-2 yr^-1 | Dead plant accumulation / g C m^-2 yr^-1 |
|---|---|---|---|
| Before re-flooding | 420 | 560 | 40 |
| After re-flooding | 620 | 330 | 150 |
Define primary production.
Using the table, determine whether the restored wetland is a carbon sink or a carbon source.
Discuss how re-flooding could change the wetland from a carbon source to a carbon sink.
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The Keeling Curve records atmospheric carbon dioxide concentration at Mauna Loa Observatory. The graph shows annual fluctuations superimposed on a long-term trend.

Describe two patterns visible in the Keeling Curve.
Explain the main biological cause of the annual fluctuation.
Explain why the long-term trend cannot be accounted for by seasonal photosynthesis alone.
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Iron-rich acidic springs contain iron-oxidizing bacteria even in shaded channels where photosynthesis is very limited. The bacteria fix carbon dioxide and obtain energy by oxidizing iron(II) ions to iron(III) ions.
State the term used for organisms that synthesize carbon compounds from inorganic substances using energy from inorganic chemical reactions.
Compare photoautotrophs and chemoautotrophs.
Evaluate the generalization that sunlight is the principal source of energy sustaining ecosystems.
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A teacher asks students to model carbon cycling in a small woodland that contains trees, herbivorous insects, birds, fungi and dead leaf litter.
Identify two carbon pools that should be included in the model.
State the process that transfers carbon from atmospheric carbon dioxide to tree biomass.
Draw a carbon cycle diagram for the woodland, showing photosynthesis, feeding, respiration and decomposition.
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A region contains natural forest, peatland and a coal-fired power station. During a drought, lightning starts a forest fire. In the same year, drained peat is burned for land clearance and coal continues to be burned for electricity.
Explain why combustion of biomass, peat and coal releases carbon dioxide.
State one way in which peat differs from recently formed biomass as a carbon store.
Evaluate why human combustion of peat and coal has a greater long-term effect on atmospheric carbon dioxide than a single natural forest fire.
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Two sealed terraria are prepared with the same mass of plants and soil. One contains an active community of fungi and bacteria in the soil. The other soil has been sterilized before planting. Both terraria receive the same light for one year.

Predict which terrarium is more likely to maintain plant growth over the year.
Explain the prediction in terms of nutrient recycling.
Evaluate the statement that only carbon needs to be recycled in ecosystems because energy enters continuously as light.
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Ecologists compared two aquatic food chains. Chain A has phytoplankton, zooplankton, small fish and large predatory fish. Chain B has aquatic plants, insect larvae and predatory birds. Energy available at each trophic level was estimated.
| Trophic level | Chain A organism | Chain A energy / kJ m^-2 yr^-1 | Chain B organism | Chain B energy / kJ m^-2 yr^-1 |
|---|---|---|---|---|
| 1 (producers) | Phytoplankton | 10000 | Aquatic plants | 10000 |
| 2 (primary consumers) | Zooplankton | 1000 | Insect larvae | 1000 |
| 3 (secondary consumers) | Small fish | 100 | Predatory birds | 100 |
| 4 (tertiary consumers) | Large predatory fish | 10 | — | — |
Using the visual, identify which food chain has more trophic levels.
Explain why energy loss restricts the number of trophic levels in ecosystems.
Discuss why apex predators often occur at low population densities or need large territories.
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A closed experimental chamber contains algae, aquatic snails and aerobic bacteria in pond water. Light intensity is varied over several days while dissolved oxygen and dissolved carbon dioxide are monitored.
| Time / h | Light intensity / % | Dissolved O2 / mg L^-1 | Dissolved CO2 / mg L^-1 |
|---|---|---|---|
| 0 | 90 | 8.2 | 6.8 |
| 6 | 90 | 9.0 | 6.1 |
| 12 | 0 | 8.6 | 6.5 |
| 18 | 0 | 8.3 | 6.8 |
| 24 | 85 | 9.1 | 6.0 |
| 30 | 85 | 9.9 | 5.3 |
| 36 | 0 | 9.6 | 5.7 |
| 42 | 0 | 9.2 | 6.1 |
| 48 | 95 | 10.0 | 5.4 |
| 54 | 95 | 10.8 | 4.7 |
| 60 | 0 | 10.5 | 5.1 |
| 66 | 0 | 10.1 | 5.5 |
| 72 | 90 | 10.9 | 4.8 |
Explain why dissolved oxygen increases during illuminated periods.
Explain why dissolved carbon dioxide increases during dark periods.
Discuss the mutual dependence of photosynthesis and aerobic respiration in ecosystems.
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