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Back to C: Interaction and interdependence

C4.2 Transfers of energy and matter

Practice exam-style IB Biology questions for Transfers of energy and matter, aligned with the syllabus and grouped by topic.

Question 1
SL • Paper 1A
Easy
Non Calculator

A pond ecosystem is considered an open system. What can cross the boundary of this ecosystem?

A.

Both energy and matter

B.

Neither energy nor matter

C.

Energy only

D.

Matter only

Question 2
SL • Paper 1A
Easy
Non Calculator

In a food chain, grass → rabbit → fox, what does the arrow from grass to rabbit represent?

A.

The rabbit is eaten by the grass

B.

Chemical energy in grass passes to the rabbit

C.

The rabbit passes heat energy to the grass

D.

Carbon dioxide diffuses from the grass to the rabbit

Question 3
SL • Paper 1A
Easy
Non Calculator

What is the immediate energy source for saprotrophic fungi growing on fallen leaves?

A.

Heat released from the surrounding soil

B.

Chemical energy in carbon compounds in dead organic matter

C.

Inorganic nitrate ions in the leaf litter

D.

Light absorbed by chlorophyll in fungal hyphae

Question 4
SL • Paper 1A
Easy
Non Calculator

What correctly describes an autotroph?

A.

It synthesizes carbon compounds from simple inorganic substances using an external energy source.

B.

It obtains carbon compounds only by ingesting other organisms.

C.

It occupies only the highest trophic level in a food chain.

D.

It releases enzymes onto dead organic matter and absorbs the products.

Question 5
SL • Paper 1A
Easy
Non Calculator

Iron-oxidizing bacteria are chemoautotrophs. What supplies the energy they use for carbon fixation?

A.

Digestion of proteins from dead animals

B.

Combustion of fossil fuels in oxygen

C.

Oxidation of iron(II) ions to iron(III) ions

D.

Absorption of visible light by chlorophyll

Question 6
SL • Paper 2
Easy
Calculator
1.

State one example of energy entering a forest ecosystem.

[1]
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2.

Outline two ways in which matter can leave the same forest ecosystem.

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Question 7
SL • Paper 2
Easy
Calculator

Distinguish between photoautotrophs and chemoautotrophs. [2]

Question 8
SL • Paper 1A
Medium
Non Calculator

A caterpillar feeds on oak leaves and is then eaten by a blue tit. What trophic level is the blue tit in this food chain?

A.

Producer

B.

Secondary consumer

C.

Tertiary consumer

D.

Primary consumer

Question 9
SL • Paper 1A
Medium
Non Calculator

What is a cause of reduced energy availability at successive trophic levels?

A.

Energy content per gram of tissue always decreases at higher trophic levels.

B.

All biomass at one trophic level is consumed by the next level.

C.

Some ingested material is egested without being absorbed.

D.

Respiration converts carbon dioxide into food biomass.

Question 10
HL • Paper 1A
Medium
Non Calculator

A fungus secretes protease onto dead wood containing animal remains and absorbs amino acids. What process follows absorption to make fungal proteins?

A.

Assimilation of absorbed amino acids into fungal biomass

B.

Egestion of amino acids as faeces

C.

Carbon fixation from atmospheric carbon dioxide

D.

Combustion of amino acids to form peat

Question 11
HL • Paper 1A
Medium
Non Calculator

In a carbon cycle diagram, which flux transfers carbon from atmospheric carbon dioxide to producer biomass?

A.

Respiration

B.

Photosynthesis

C.

Egestion

D.

Feeding

Question 12
SL • Paper 2
Medium
Calculator

A community contains phytoplankton, water fleas, small fish and herons. Small fish feed on water fleas. Herons feed on small fish. Water fleas feed on phytoplankton.

1.

Construct a food chain using these organisms.

[1]
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2.

State what the arrows in the food chain represent.

[1]
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Question 13
SL • Paper 2
Medium
Calculator

Explain why not all chemical energy in producer biomass is transferred to primary consumers. [3]

Question 14
SL • Paper 2
Medium
Calculator

A student burns dry plant material beneath a tube containing water to estimate the energy content of the biomass.

A simple calorimetry apparatus with dry biomass burning beneath a water-filled tube or boiling tube, a thermometer in the water, and labels for biomass, water, flame and thermometer.
1.

State the temperature change needed for the calculation.

[1]
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2.

Outline two reasons why this method may underestimate the true energy content of the biomass.

[1]
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Question 15
SL • Paper 2
Medium
Calculator
1.

Define primary production.

[1]
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2.

Outline how net primary production differs from gross primary production.

[1]
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Question 16
SL • Paper 1B
Medium
Non Calculator

The graph shows the energy available at successive trophic levels in a grassland food chain.

Bar chart showing energy available at four trophic levels in a grassland food chain.
1.

Identify the trophic level with the greatest energy available.

[1]
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2.

Describe the trend in energy availability through the food chain.

[1]
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3.

Calculate the percentage transfer of energy from producers to primary consumers using the values shown.

[1]
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4.

Explain one reason for the low percentage transfer between these trophic levels.

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Question 17
SL • Paper 1B
Medium
Non Calculator

The graph shows light intensity with depth at two lake sites and the measured rate of photosynthesis by phytoplankton.

Relative light intensity and phytoplankton photosynthesis at depth in clear-water and turbid-water lake sites.
1.

State how light intensity changes with depth at both sites.

[1]
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2.

Compare the depth at which photosynthesis becomes very low in the clear-water and turbid-water sites.

[1]
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3.

Suggest why primary production is lower in the turbid-water site.

[1]
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Question 18
SL • Paper 1A
Medium
Non Calculator

A wetland is a carbon sink over one year. What must be true for that year?

A.

Feeding transfers carbon directly from consumers to atmospheric carbon dioxide.

B.

Respiration releases more carbon dioxide than photosynthesis removes.

C.

Combustion releases carbon dioxide faster than peat accumulates.

D.

Photosynthesis removes more carbon dioxide than respiration releases.

Question 19
HL • Paper 1A
Medium
Non Calculator

Why can energy not be recycled through an ecosystem in the same way as chemical elements?

A.

Heat produced in respiration cannot be gathered by organisms to rebuild chemical energy in food chains.

B.

Atoms of carbon are converted into atoms of oxygen during feeding.

C.

Photosynthesis produces heat but no chemical energy.

D.

Energy is destroyed when organisms respire.

Question 20
HL • Paper 1A
Medium
Non Calculator

What units are most suitable for measuring primary production in a terrestrial biome?

A.

g C m⁻² yr⁻¹

B.

cm³ O₂ g⁻¹

C.

mol CO₂ dm⁻³

D.

kJ kg⁻¹ °C⁻¹

Question 21
HL • Paper 1A
Medium
Non Calculator

Why is secondary production usually lower than primary production in an ecosystem?

A.

Heterotrophs convert some carbon compounds into carbon dioxide and water during respiration.

B.

Heterotrophs fix more carbon dioxide than autotrophs.

C.

Consumers have no anabolic reactions.

D.

Autotrophs never respire carbon compounds.

Question 22
HL • Paper 1A
Medium
Non Calculator

What mainly causes the long-term upward trend in the Keeling Curve?

A.

Seasonal photosynthesis in northern hemisphere forests

B.

Combustion of fossil fuels and land-use change

C.

Conversion of atmospheric carbon dioxide into peat every winter

D.

Daily changes in oxygen concentration at Mauna Loa

Question 23
HL • Paper 1A
Medium
Non Calculator

What describes a major interaction between photosynthesis and aerobic respiration at a global scale?

A.

Photosynthesis uses oxygen released by aerobic respiration.

B.

Aerobic respiration depends on oxygen maintained by photosynthesis, while photosynthesis depends on carbon dioxide released by respiration.

C.

Photosynthesis and respiration both recycle heat into chemical energy.

D.

Aerobic respiration fixes carbon dioxide into glucose for plants.

Question 24
HL • Paper 1A
Medium
Non Calculator

What is the key reason matter can be recycled in ecosystems?

A.

Energy released as heat is converted back into producer biomass by decomposers.

B.

All elements are permanently locked in living organisms.

C.

Consumers create new atoms during assimilation.

D.

Atoms of chemical elements are conserved and can move between biotic and abiotic pools.

Question 25
SL • Paper 2
Medium
Calculator

The boxes in a carbon cycle diagram are labelled: atmospheric carbon dioxide, producer biomass, consumer biomass and dead organic matter.

1.

Identify the process transferring carbon from atmospheric carbon dioxide to producer biomass.

[1]
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2.

Identify the process transferring carbon from producer biomass to consumer biomass.

[1]
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3.

Explain how carbon in dead organic matter can return to atmospheric carbon dioxide.

[1]
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Question 26
SL • Paper 2
Medium
Calculator

Explain why food chains rarely contain more than four or five trophic levels. [4]

Question 27
HL • Paper 2
Medium
Calculator

A python digests a swallowed rat over several days.

1.

State why proteins and nucleic acids in the rat must be digested before absorption.

[1]
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2.

Explain how digestion and assimilation allow secondary production in the python.

[1]
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Question 28
HL • Paper 2
Medium
Calculator

A deep-ocean community occurs below the depth reached by light. Tube worms live near vents where reduced inorganic chemicals are released.

1.

State why photosynthesis cannot be the local energy source for producers in this community.

[1]
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2.

Suggest how carbon compounds can still be produced in this community.

[1]
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Question 29
HL • Paper 2
Medium
Calculator

A drained peatland is rewetted as part of a restoration project.

1.

State the condition under which an ecosystem is a carbon sink.

[1]
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2.

Explain why waterlogged peat can sequester carbon.

[1]
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Question 30
HL • Paper 2
Medium
Calculator

A 0.80 g dry seed sample is completely burned beneath 50.0 g of water. The water temperature rises by 18.0 K. Use Eᵦ = (m𝓌cΔT) / mᵦ and c = 4.18 J g⁻¹ K⁻¹.

1.

Calculate the energy released by the seed sample.

[1]
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2.

Calculate the energy content in kJ g⁻¹.

[1]
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Question 31
HL • Paper 2
Medium
Calculator

Outline why combustion of coal has a different carbon-cycle significance from combustion of recently grown wood. [3]

Question 32
HL • Paper 2
Medium
Calculator

Explain the dependence of aerobic organisms on photosynthesizing organisms and the dependence of photosynthesis on respiration. [4]

Question 33
SL • Paper 1B
Medium
Non Calculator

A forest was monitored for carbon dioxide exchange over one year. Negative net exchange indicates uptake of CO₂ by the forest, and positive net exchange indicates release of CO₂.

Monthly net ecosystem CO2 exchange for a temperate forest over one year.
1.

Identify the season in which the forest acts most strongly as a carbon sink.

[1]
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2.

State one month or period when the forest acts as a carbon source.

[1]
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3.

Explain how photosynthesis and respiration determine whether the forest is a sink or a source.

[1]
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4.

Suggest one reason why the forest may release CO₂ during winter.

[1]
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Question 34
SL • Paper 1B
Medium
Non Calculator

The food web shows feeding relationships in a rocky shore community.

A food web diagram for a rocky shore with labelled organisms such as seaweed, phytoplankton, limpets, mussels, small crabs, whelks, shore fish and gulls. Arrows point from food to feeder, and at least one omnivore feeds on both producers and consumers.
1.

Identify one producer in the food web.

[1]
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2.

Identify one organism that can act as a secondary consumer in one food chain.

[1]
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3.

Explain why one named organism in the web may occupy different trophic levels.

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Question 35
SL • Paper 1B
Medium
Non Calculator

A student used simple combustion calorimetry to estimate energy content of three types of dry biomass.

Biomass sampleMass burned / gWater mass / gInitial temp / °CFinal temp / °CTemp rise / °C
Wood shavings0.8010021.041.420.4
Dry leaves0.7510021.236.315.1
Rice husk0.9010021.139.518.4
1.

Identify the biomass sample with the highest estimated energy content.

[1]
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2.

Calculate the energy content of one named sample using the data in the table and the formula Eᵦ = (m𝓌cΔT) / mᵦ.

[1]
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3.

State one variable that should be controlled when comparing the samples.

[1]
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4.

Suggest why all estimates are likely to be lower than the true energy contents.

[1]
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Question 36
HL • Paper 2
Medium
Calculator

Explain the two main features of the Keeling Curve: the annual fluctuation and the long-term increase. [4]

Atmospheric CO₂ concentration measured at Mauna Loa from 1960 to 2024.
Question 37
HL • Paper 2
Medium
Calculator

Explain the role of decomposers in the recycling of chemical elements in ecosystems. [4]

Question 38
HL • Paper 1B
Hard
Non Calculator

The graph shows atmospheric CO₂ concentration measured at two monitoring stations: one remote oceanic station and one station near a large forested land mass.

Quarterly atmospheric CO₂ concentrations at two monitoring stations from 2000 to 2020.
1.

Compare the annual fluctuations at the two stations.

[1]
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2.

Explain the long-term trend common to both stations.

[1]
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3.

Suggest why remote monitoring stations are useful for estimating global atmospheric CO₂.

[1]
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Question 39
HL • Paper 1B
Hard
Non Calculator

The table compares net primary production in four biomes.

BiomeNPP / g C m⁻² yr⁻¹
Tropical rainforest2200
Temperate grassland600
Tundra140
Desert90
1.

Identify the biome with the highest net primary production.

[1]
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2.

Calculate how many times greater the highest value is than the lowest value.

[1]
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3.

Suggest two abiotic factors that could explain differences in net primary production among the biomes.

[1]
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4.

State the correct units for net primary production shown in the table.

[1]
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Question 40
HL • Paper 1B
Hard
Non Calculator

Iron-oxidizing bacteria were grown in acidic water containing iron(II) ions. The graph shows changes in iron(II) concentration and bacterial biomass over time.

Changes in normalized iron(II) concentration and bacterial biomass during growth in acidic water.
1.

Describe the change in iron(II) concentration over time.

[1]
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2.

Describe the change in bacterial biomass over time.

[1]
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3.

Suggest how the bacteria obtain energy for increasing biomass.

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Question 41
SL • Paper 2
Hard
Calculator
1.

Outline how chemical energy enters and moves through a simple grazing food chain.

[1]
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2.

Explain why energy flow through the food chain is not the same as recycling of matter.

[1]
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Question 42
SL • Paper 2
Hard
Calculator
1.

Construct a possible food chain from the following organisms: algae, mayfly larva, trout, kingfisher.

[1]
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2.

Discuss why a food web is a more useful model than a single food chain for representing a community.

[1]
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Question 43
HL • Paper 1B
Hard
Non Calculator

The diagram shows energy and carbon transfers for a population of herbivorous insects feeding on leaves.

TransferDescriptionDirectionCarbon flux / g C m⁻² yr⁻¹
ALeaf carbon consumedleaves → insects120
BFaecal carboninsects → faeces65
CRespiratory carbon lossinsects → CO₂38
1.

Identify the input to the insect population that represents ingestion.

[1]
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2.

Calculate assimilation using the values shown for ingestion and egestion.

[1]
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3.

Calculate secondary production using the values shown for assimilation and respiration.

[1]
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4.

Explain why secondary production is lower than ingestion.

[1]
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Question 44
HL • Paper 1B
Hard
Non Calculator

The table shows estimated carbon dioxide released by combustion of different carbon stores in one region during a dry year.

Carbon storeMain ignition or useCO2 release / Mt yr⁻¹
Recent biomassWildfire and crop burning18.4
PeatDrained peatland fires46.7
CoalElectricity generation32.5
OilTransport fuels21.8
Natural gasHeating and industry12.6
1.

Identify the store that contributed the greatest carbon dioxide release.

[1]
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2.

Compare the likely time scales over which carbon in biomass and peat accumulated before combustion.

[1]
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3.

Suggest why human activity can increase carbon dioxide release from peatlands.

[1]
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4.

Explain why combustion is a carbon flux to the atmosphere.

[1]
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Question 45
SL • Paper 2
Hard
Calculator
1.

State two features of a correctly constructed energy pyramid.

[1]
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2.

Explain why energy pyramids usually narrow at higher trophic levels and how this restricts food-chain length.

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Question 46
SL • Paper 2
Hard
Calculator
1.

Define biomass and primary production.

[1]
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2.

Compare and contrast primary production and secondary production in ecosystems.

[1]
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Question 47
HL • Paper 2
Hard
Calculator
1.

Outline two biological carbon fluxes that should be included in a carbon cycle diagram.

[1]
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2.

Discuss how ecosystems can act as carbon sinks or carbon sources, including the roles of photosynthesis, respiration, decomposition and combustion.

[1]
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Question 48
HL • Paper 2
Hard
Calculator
1.

State two patterns shown by the Keeling Curve.

[1]
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2.

Evaluate the explanation that the annual fluctuation and long-term trend in atmospheric CO₂ have the same cause.

[1]
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Question 49
HL • Paper 2
Hard
Calculator
1.

Distinguish between autotrophs and heterotrophs.

[1]
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2.

Explain how autotrophs and heterotrophs obtain, transform and use carbon compounds and energy in ecosystems.

[1]
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Question 50
HL • Paper 2
Hard
Calculator
1.

Outline why all living organisms require a continuous supply of chemical elements.

[1]
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2.

Discuss why matter can be recycled in ecosystems but energy cannot.

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C4.1 Populations and communities