Two groups of the same limpet species live on rocky shores separated by a long sandy beach. Adults remain on their own shore and larvae rarely disperse across the sandy area. What is the best reason for treating the two groups as separate populations?
They occupy different trophic levels.
They are reproductively isolated from each other.
They belong to different communities.
They have different abiotic requirements.
An ecologist estimates the number of small plants in a meadow using quadrats placed at coordinates generated by a random number table. What is the main reason for using random coordinates?
To ensure every possible sampling location has an equal chance of selection
To ensure the estimate is identical to the true population size
To avoid calculating a mean from the quadrat counts
To allow only the most typical-looking areas to be included
A freshwater pond contains water lilies, algae, fish, snails, fungi and bacteria that interact with each other. The pH, temperature and dissolved mineral ions of the pond are not included in the description. What ecological term best describes the living organisms listed?
An ecosystem
A population
A community
A habitat
Ten randomly placed quadrats, each with an area of , contain a total of 80 barnacles. The rocky shore being estimated has an area of . What is the estimated barnacle population size?
8000
320
3200
800
In a capture-mark-release-recapture study of field mice, 40 mice are captured, marked and released. Later, 50 mice are captured and 10 of these are marked. What population size is estimated by the Lincoln index?
200
500
100
80
A rabbit population increases above the carrying capacity of a grassland. Crowding then increases transmission of a viral disease, reducing survival and returning the population closer to the carrying capacity. What type of regulation is described?
Reproductive isolation
Random sampling error
Density-dependent negative feedback
Density-independent positive feedback
Black walnut trees release juglone into the soil, inhibiting growth of some neighbouring plants. Some Penicillium fungi release penicillin-like compounds that inhibit susceptible bacteria. What feature is shared by these interactions?
A chemical is released into the environment to reduce potential competitors.
Both species benefit by exchanging resources.
A predator kills and eats prey immediately.
A host is infected by a pathogen causing disease.
Limpets of the same species live on two rocky shores separated by a wide sandy bay. Larvae from one rocky shore rarely survive long enough to reach the other shore.
Define a population.
Explain why the limpets on the two rocky shores could be regarded as separate populations.
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A yeast population is plotted with population size on a logarithmic vertical axis and time on a non-logarithmic horizontal axis. During the first eight hours the points lie close to a straight line. What does this pattern indicate for this part of the growth curve?
The population is limited only by density-independent factors.
The population has reached carrying capacity.
The population is growing exponentially.
The population is decreasing at a constant numerical rate.
In a field manipulation, researchers remove barnacle species B from randomly selected plots but leave it in control plots. Barnacle species A increases in abundance only in the removal plots. What hypothesis is most directly supported?
Species A depends on species B for reproduction.
Species B competes with species A for a limiting resource.
Species B is controlled by density-independent factors only.
Species A and species B are the same population.
In reef-building corals, zooxanthellae live inside coral cells. What pair of benefits correctly describes this mutualism?
The algae kill coral pathogens; the coral releases antibiotics into seawater.
The algae receive prey; the coral receives mineral ions from fungal hyphae.
The algae receive fixed nitrogen; the coral receives ammonium from root nodules.
The algae receive shelter and carbon dioxide; the coral receives organic compounds from photosynthesis.
In a coastal community, sea otters reduce sea urchin numbers. Reduced grazing by sea urchins allows kelp biomass to increase. What type of population control is shown by the effect of otters on kelp?
Top-down control
Bottom-up control
Reproductive isolation control
Random sampling control
A student estimated the population size of marram grass clumps in a sand dune. A quadrat was placed at five random coordinates in a study area. The numbers of clumps in the quadrats were 3, 1, 4, 0 and 2.
Calculate the mean number of marram grass clumps per quadrat.
Calculate the estimated population size of marram grass clumps in the whole study area.
State what a high standard deviation for the quadrat counts would indicate about the distribution of marram grass.
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A population of woodlice was estimated using capture-mark-release-recapture. In the first sample, 48 woodlice were captured, marked harmlessly and released. In the second sample, 60 woodlice were captured, of which 12 were marked.
Calculate the population size using the Lincoln index.
Outline two assumptions made when using this method.
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Aphids feeding on bean plants reproduce rapidly when first introduced into a greenhouse. After several weeks, the aphid population fluctuates around a relatively stable size.
Define carrying capacity.
Explain how density-dependent factors can act as negative feedback on the aphid population.
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Duckweed was grown in nutrient solution in identical containers. The graph shows the number of duckweed fronds over time.

State one feature of the graph that shows sigmoid population growth.
Explain why the rate of increase becomes slower near the plateau.
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A shallow reef contains hard corals, zooxanthellae, grazing fish, predatory fish, fungi and bacteria. Abiotic factors include light intensity, temperature and salinity.
Distinguish between a community and an ecosystem in this reef.
Outline the mutualism between hard corals and zooxanthellae.
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Black walnut trees release juglone into the soil. Penicillium fungi release penicillin-like compounds into their surroundings.
Outline how juglone release can reduce competition around black walnut trees.
State one similarity between allelopathy and antibiotic secretion.
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Ecologists estimated the population size of a sessile coastal plant, sea rocket (Cakile maritima), on a sand-dune area. A grid was marked over the habitat and ten random coordinates were selected. A quadrat of area was placed at each coordinate.
| Quadrat | Count of plants |
|---|---|
| 1 | 5 |
| 2 | 3 |
| 3 | 7 |
| 4 | 4 |
| 5 | 6 |
| 6 | 4 |
| 7 | 5 |
| 8 | 6 |
| 9 | 5 |
| 10 | 4 |
Calculate the estimated population size of sea rocket plants in the whole habitat. Show your working.
The standard deviation for the quadrat counts was . Outline what this indicates about the distribution of sea rocket in the sampled area.
Explain why random coordinates were used instead of placing quadrats where plants looked most abundant.
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A population of ground beetles in a woodland was estimated using capture-mark-release-recapture. Beetles were marked with a small harmless dot of paint and released at the capture site. A second capture was made three days later.
| Sample | Marked (beetles) | Unmarked (beetles) | Total (beetles) |
|---|---|---|---|
| First capture (Day 0) | 80 | 0 | 80 |
| Second capture (Day 3) | 12 | 48 | 60 |
Calculate the estimated population size using the Lincoln index.
Suggest one reason why a longer time between release and recapture could improve the estimate.
student suggested that marked beetles may be more visible to predators. Explain how this would affect the estimate.
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Hard corals contain photosynthetic algae called zooxanthellae in their cells. Researchers measured coral growth and the density of zooxanthellae under two temperature treatments.
| Temperature treatment | Coral growth / mm month^-1 | Zooxanthellae density / 10^6 cells cm^-2 |
|---|---|---|
| Normal temperature | 4.6 ± 0.4 | 1.35 ± 0.10 |
| High temperature | 2.0 ± 0.3 | 0.62 ± 0.07 |
State the type of interspecific relationship between hard corals and zooxanthellae.
Using the data, describe the effect of high temperature on the coral-zooxanthellae association.
Suggest why a decrease in zooxanthellae density reduces coral growth.
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A chi-squared test for association between two plant species gives . The test uses a presence-absence table, so . The critical value at is 3.84. What conclusion is supported?
Reject the null hypothesis; there is evidence of an association between the species.
Accept the null hypothesis; the calculated value is statistically significant.
Accept the null hypothesis; the species must be distributed independently.
Reject the null hypothesis; interspecific competition has been proven.
Field observations show that seaweed species A is abundant on rocks where seaweed species B is absent, but species A is rare where species B is present.
Explain why these observations alone do not prove interspecific competition.
Suggest a field manipulation to test whether species B reduces the abundance of species A.
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Historical records from northern forests show repeated cycles in snowshoe hare and Canada lynx populations. Hare numbers usually increase before lynx numbers increase.

State why the lynx population peak occurs after the hare population peak.
Explain how this predator-prey relationship can act as density-dependent control of hare population size.
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In a kelp forest, sea otters feed on sea urchins, and sea urchins graze kelp. In some years, nitrate availability in seawater is low.

Identify the type of control shown when sea otters reduce sea urchin numbers and kelp increases.
Explain how low nitrate availability could cause bottom-up control in this community.
Suggest one type of data that could help decide which control is dominant in a particular year.
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In some New Zealand beech forests, introduced common wasps feed heavily on honeydew produced by scale insects. Honeydew is also used as food by endemic birds and insects.
Define an endemic species and an invasive species.
Explain how common wasps can reduce populations of endemic honeydew-feeding species.
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Duckweed fronds were grown in identical trays of nutrient solution under controlled light and temperature. The number of fronds was counted every two days.

Identify the approximate carrying capacity shown by the duckweed culture.
Describe the change in growth rate during the experiment.
Explain why the duckweed population did not continue to increase exponentially.
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A deer population on an island was monitored for six years. Researchers recorded deer density, prevalence of a parasitic nematode and survival of fawns to one year old.

Describe the relationship between deer density and parasite prevalence.
Explain why parasitism in this example is density-dependent.
Suggest how the parasite could contribute to negative feedback control of deer population size.
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Four pond mesocosms were set up with equal volumes of pond water containing phytoplankton and zooplankton. Some mesocosms received extra phosphate and some received a fish predator that feeds on zooplankton. Phytoplankton biomass was measured after ten days.

Identify which treatment provides evidence for bottom-up control of phytoplankton.
Explain why adding fish could increase phytoplankton biomass.
Compare the evidence for top-down and bottom-up control in this mesocosm community.
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Two investigations tested chemical interactions between organisms. In one, tomato seedlings were grown in soil containing different concentrations of juglone from black walnut. In the other, paper discs containing filtrate from Penicillium cultures were placed on agar seeded with bacteria.
| Bioassay | Treatment | Tomato seedling root length / cm | Bacterial inhibition zone / mm |
|---|---|---|---|
| Tomato seedlings | 0 mg L^-1 juglone | 8.5 | — |
| Tomato seedlings | 1 mg L^-1 juglone | 6.8 | — |
| Tomato seedlings | 2 mg L^-1 juglone | 4.2 | — |
| Tomato seedlings | 4 mg L^-1 juglone | 1.8 | — |
| Bacteria | Sterile disc | — | 0 |
| Bacteria | Penicillium filtrate disc | — | 18 |
Describe the effect of juglone on tomato seedlings.
Explain how secretion of penicillin-like compounds could benefit Penicillium in soil.
Compare allelopathy and secretion of antibiotics as ecological interactions.
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Emperor penguin chicks form crèches while adults forage at sea. Researchers recorded chick survival and mean body mass change in groups of different sizes during a period of cold weather and limited food delivery.
| Crèche size (chicks) | Chick survival (%) | Mean body mass change (g) |
|---|---|---|
| 1–5 | 61 | -68 |
| 6–10 | 70 | -54 |
| 11–15 | 86 | -39 |
| 16–20 | 79 | -73 |
| 21–25 | 67 | -109 |
Using the data, identify one benefit of cooperation in crèches.
Suggest why very large crèches may lead to greater loss of body mass.
Discuss how the data show both cooperation and competition within one population.
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Two barnacle species were surveyed at 30 randomly selected sites on a rocky shore. The observed counts were: both species present, 3 sites; species A only, 12 sites; species B only, 10 sites; neither species present, 5 sites. Expected frequencies for a chi-squared test of association are 6.5, 8.5, 6.5 and 8.5 respectively. The critical value of at and is 3.84.
| Site category | Observed count | Expected frequency |
|---|---|---|
| Both species present | 3 | 6.5 |
| Species A only | 12 | 8.5 |
| Species B only | 10 | 6.5 |
| Neither species present | 5 | 8.5 |
State the null hypothesis for this test.
Calculate the value of .
Conclude whether there is evidence of an association between the two species.
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In a New Zealand beech forest, introduced wasps feed on honeydew produced by scale insects. Honeydew is also used by endemic birds and insects. Researchers measured wasp density and the proportion of available honeydew removed during summer.

Identify the shared resource in this example.
Using the data, suggest why introduced wasps may reduce populations of endemic honeydew-feeding species.
Suggest one feature of the introduced wasps that could give them a competitive advantage in resource acquisition.
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Historical trapping records have been used as an indirect measure of Canada lynx and snowshoe hare population sizes in northern forests. The graph shows relative abundance over several cycles.

Describe the timing of changes in lynx abundance compared with hare abundance.
Explain how this predator-prey relationship can produce density-dependent control of the hare population.
Suggest why predator-prey interaction alone may not fully explain the hare cycle.
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The spread of an introduced bird population was monitored for eight years after colonization of a region. The same data were plotted on two graphs: one with a linear vertical axis and one with a logarithmic vertical axis.

State the evidence from the logarithmic graph that early growth was approximately exponential.
Explain why exponential growth is expected during the early phase after colonization.
Evaluate the usefulness of the exponential growth model for the later years of the study.
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A population of a sessile shore plant was surveyed in a rectangular salt marsh. Students used random coordinates to place quadrats and counted the number of plants in each quadrat.
| Item | Value |
|---|---|
| Salt marsh length / m | 25 |
| Salt marsh width / m | 16 |
| Quadrat area / m² | 1 |
| Quadrat 1 / plants | 0 |
| Quadrat 2 / plants | 7 |
| Quadrat 3 / plants | 1 |
| Quadrat 4 / plants | 11 |
| Quadrat 5 / plants | 0 |
| Quadrat 6 / plants | 4 |
| Quadrat 7 / plants | 2 |
| Quadrat 8 / plants | 6 |
| Quadrat 9 / plants | 1 |
| Quadrat 10 / plants | 9 |
| Quadrat 11 / plants | 4 |
| Quadrat 12 / plants | 3 |
| Standard deviation / plants | 3.6 |
Use the data to estimate the population size of the shore plant in the salt marsh.
Calculate the number of quadrat areas that fit into the whole habitat.
Use the mean number per quadrat to calculate the estimated population size.
Explain why random sampling is needed when estimating the population size of this plant.
Evaluate the reliability of the estimate using the standard deviation shown in the data.
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A conservation team estimated the population size of a motile lizard in a dry woodland using capture-mark-release-recapture. Lizards were marked with a small non-toxic paint spot before release.
| Variable | Meaning | Count / lizards |
|---|---|---|
| M | lizards marked and released in first sample | 24 |
| N | total lizards caught in second sample | 40 |
| R | marked lizards recaptured in second sample | 10 |
Use the Lincoln index to estimate the population size of the lizard.
State the formula for the Lincoln index using the symbols , and .
Calculate the estimated population size using the data.
Explain two assumptions of capture-mark-release-recapture that must be met for this estimate to be valid.
Discuss how the heavy rain between sampling days could affect the validity of the estimate.
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Meerkats live in groups in which individuals forage for insects, compete for breeding opportunities and sometimes produce alarm calls when predators approach.
Compare competition and cooperation as intraspecific relationships in the meerkat population.
State why competition between meerkats is described as intraspecific.
Outline one reason why intraspecific competition can be intense.
Explain how cooperation could increase survival in a meerkat population.
Discuss why the same population should not be described as only competitive or only cooperative.
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Black walnut trees release juglone into the surrounding soil. Some fungi such as Penicillium release antibiotic compounds into soil where bacteria are growing.

Outline the effect of each released chemical on potential competitors.
Outline the effect of juglone released by black walnut.
Outline the effect of antibiotics released by Penicillium.
Compare and contrast allelopathy and antibiotic secretion as ecological interactions.
Discuss why these interactions can affect community structure.
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Two barnacle species were recorded as present or absent in 100 randomly selected quadrats on a rocky shore. A chi-squared test was used to test for association between the species. The critical value at with degree of freedom is .
| Species 2 present | Species 2 absent | Total | |
|---|---|---|---|
| Species 1 present | 5 | 45 | 50 |
| Species 1 absent | 25 | 25 | 50 |
| Total | 30 | 70 | 100 |
Calculate the expected frequency for quadrats in which both species are present.
The calculated chi-squared value is . State the conclusion of the chi-squared test.
Evaluate whether these data prove interspecific competition between the two barnacle species.
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A population of freshwater snails in a pond fluctuates around a long-term average. In summer, snail density increases rapidly, followed by a rise in a fungal disease and increased predation by ducks.

Distinguish between density-dependent and density-independent factors affecting population size.
State what is meant by a density-dependent factor.
Give one example of a density-independent factor.
Explain how negative feedback could regulate the snail population when it rises above carrying capacity.
Explain why carrying capacity should not be treated as a fixed value in this pond.
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Duckweed fronds were grown in identical trays of nutrient solution. The number of fronds was recorded every two days to model population growth under controlled conditions.
| Day / d | Fronds / count | log10(fronds) |
|---|---|---|
| 0 | 4 | 0.60 |
| 2 | 6 | 0.78 |
| 4 | 10 | 1.00 |
| 6 | 16 | 1.20 |
| 8 | 26 | 1.41 |
| 10 | 42 | 1.62 |
| 12 | 64 | 1.81 |
| 14 | 91 | 1.96 |
| 16 | 120 | 2.08 |
| 18 | 145 | 2.16 |
| 20 | 164 | 2.21 |
| 22 | 177 | 2.25 |
| 24 | 186 | 2.27 |
| 26 | 191 | 2.28 |
| 28 | 194 | 2.29 |
| 30 | 195 | 2.29 |
Use the graphs to identify the growth model that best describes the whole investigation.
Identify the shape of the population growth curve shown for the whole investigation.
State the phase represented by the plateau region.
Explain why the initial part of the duckweed curve may approximate exponential growth.
Evaluate the use of this duckweed investigation as a model of population growth in a natural pond.
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The diagram shows three mutualistic relationships: legumes with nitrogen-fixing bacteria in root nodules, orchids with mycorrhizal fungi and hard corals with zooxanthellae.

For two of the mutualisms shown, state one benefit to each partner.
State the benefit to the plant or coral partner in one mutualism.
State the benefit to the bacterial, fungal or algal partner in the same or another mutualism.
Explain why these relationships are classified as mutualism rather than parasitism or herbivory.
Discuss how mutualisms can affect population sizes within a community.
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Ecologists observed that a native grass species was more abundant in plots where an introduced grass species was absent. They proposed that the introduced grass reduces the native grass population by interspecific competition for soil nitrate.

Distinguish between an observation and an experiment in testing this hypothesis.
State one feature of a field observation.
State one feature of an experiment.
Explain how a field manipulation could test whether the introduced grass competes with the native grass.
Discuss why several approaches may provide stronger evidence than field observation alone.
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Long-term records of snowshoe hare and Canada lynx numbers show repeated cycles in northern forests. Hare numbers generally increase before lynx numbers increase.

Use the graph to describe the relationship between the hare and lynx population cycles.
Identify which population generally peaks first.
State what happens to lynx numbers after hare numbers decline.
Explain how this predator-prey relationship can produce density-dependent control of the hare population.
Discuss why predation alone may be an incomplete explanation for the cycles.
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A coastal food chain includes sea otters, sea urchins and kelp. In some bays, sea otter numbers declined sharply. In other bays, nitrogen concentration in the water decreased after changes in ocean currents.

Distinguish between top-down and bottom-up control in this community.
State what is meant by top-down control.
State what is meant by bottom-up control.
Explain the likely effect of sea otter decline on kelp abundance.
Evaluate whether the same food-chain diagram is sufficient to predict kelp abundance in both bays.
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In a New Zealand beech forest, introduced common wasps and German wasps feed heavily on honeydew produced by scale insects. Honeydew is also an important food resource for endemic birds and insects.

Use the example to distinguish between endemic and invasive species.
State what is meant by an endemic species.
State why the wasps are described as invasive in this forest.
Explain how resource competition for honeydew could reduce endemic populations.
Discuss why the wasps may have a competitive advantage in resource acquisition.
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Two sessile barnacle species were recorded as present or absent in randomly selected quadrats on a rocky shore. The data were analysed using a chi-squared test for association.
| Category | B present | B absent | Total | Statistic value |
|---|---|---|---|---|
| A present | 5 | 15 | 20 | |
| A absent | 15 | 5 | 20 | |
| Total | 20 | 20 | 40 | |
| χ² statistic | 10.0 | |||
| Critical value (df=1, p=0.05) | 3.84 |
Apply the chi-squared test to determine whether there is evidence of an association between the two barnacle species.
State the null hypothesis for this test.
Calculate one expected frequency using .
Use the calculated p=0.05$.
Explain why a negative association between the two barnacle species may indicate interspecific competition.
Evaluate the conclusion that the chi-squared result proves competition between the barnacle species.
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