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A4.1 Evolution and speciation

Practice exam-style IB Biology questions for Evolution and speciation, aligned with the syllabus and grouped by topic.

Question 1
SL • Paper 1A
Easy
Non Calculator

A population of lizards develops darker skin after basking in strong sunlight, but their offspring raised in shade are not darker. What conclusion is supported?

A.

The darker skin is an acquired characteristic and is not evidence of evolution.

B.

The darker skin is speciation because a new lizard species has appeared.

C.

The darker skin is evolution because individuals changed in response to the environment.

D.

The darker skin is Lamarckian inheritance because offspring inherit it in shade.

Question 2
SL • Paper 1A
Easy
Non Calculator

Two mammal species have very similar amino acid sequences in the same enzyme. What is the most direct evolutionary inference?

A.

They probably share a relatively recent common ancestor.

B.

They must occupy identical ecological niches.

C.

They are unable to interbreed with other mammals.

D.

They acquired the enzyme by learning the same behaviour.

Question 3
SL • Paper 1A
Easy
Non Calculator

Selective breeding of crop plants is evidence for evolution because it shows that

A.

heritable traits in a population can change markedly over generations.

B.

individual plants can decide to develop useful characteristics.

C.

all crop varieties become separate species after one generation.

D.

wild species have no genetic variation before domestication.

Question 4
SL • Paper 1A
Easy
Non Calculator

The humerus, radius, ulna, carpals, metacarpals and phalanges occur in the same relative positions in a bat wing and a whale flipper. What does this provide evidence for?

A.

Hybrid sterility between mammals

B.

Homology due to common ancestry

C.

Acquired traits caused by swimming

D.

Analogy due to identical function

Question 5
SL • Paper 1A
Easy
Non Calculator

What must occur for speciation, rather than only gradual evolutionary change within one species?

A.

Extinction removes one population from an ecosystem.

B.

All individuals in a population become larger over time.

C.

An individual changes during its lifetime.

D.

A pre-existing species splits into two or more separate species.

Question 6
HL • Paper 1A
Easy
Non Calculator

Two populations become separate species while living in the same lake because females respond to different male courtship colours. What type of speciation is described?

A.

Allopatric speciation involving geographical isolation

B.

Autopolyploid speciation involving chromosome doubling

C.

Adaptive radiation involving temporal isolation only

D.

Sympatric speciation involving behavioural isolation

Question 7
HL • Paper 1A
Easy
Non Calculator

Adaptive radiation most directly increases biodiversity when related species

A.

diversify from a common ancestor into different ecological niches.

B.

all adapt to use the same resource in the same way.

C.

merge their gene pools through fertile hybrids.

D.

lose all inherited variation by selective breeding.

Question 8
SL • Paper 2
Easy
Calculator
1.

Define evolution.

[1]
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2.

Distinguish evolution from an acquired characteristic using one example.

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Question 9
SL • Paper 2
Easy
Calculator
1.

State what happens to the total number of species when speciation occurs.

[1]
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2.

Outline why gradual change in a single interbreeding lineage is not necessarily speciation.

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Question 10
SL • Paper 1A
Medium
Non Calculator

Bird wings and insect wings are both used for flight but have different evolutionary origins. What process best explains this similarity?

A.

Divergent evolution producing homologous structures

B.

Polyploidy producing new plant species

C.

Convergent evolution producing analogous structures

D.

Extinction reducing total species number

Question 11
SL • Paper 1A
Medium
Non Calculator

An ancestral population of apes is divided by a deep river. Food resources and competitors differ on the two sides. What combination most directly promotes speciation?

A.

Extinction and identical gene pools

B.

High gene flow and identical selection

C.

Acquired behaviours and no heritable variation

D.

Reduced gene flow and differential selection

Question 12
HL • Paper 1A
Medium
Non Calculator

Two closely related plant populations in the same meadow release pollen at different times of year, preventing fertilization between them. What isolation mechanism is shown?

A.

Temporal isolation

B.

Hybrid sterility

C.

Geographic isolation

D.

Adaptive radiation

Question 13
HL • Paper 1A
Medium
Non Calculator

Male frogs of two species call at the same pond, but females mate only with males producing their species-specific call. What type of reproductive barrier is this?

A.

A prezygotic behavioural barrier

B.

A polyploid chromosome barrier

C.

A geographical barrier

D.

A postzygotic sterility barrier

Question 14
HL • Paper 1A
Medium
Non Calculator

A mule is usually sterile because horse and donkey chromosomes do not pair correctly during meiosis. What is the evolutionary effect of this sterility?

A.

It is a prezygotic barrier preventing fertilization.

B.

It prevents most allele flow from hybrids into later generations.

C.

It increases gene flow between horses and donkeys.

D.

It proves that horses and donkeys have identical gene pools.

Question 15
HL • Paper 1A
Medium
Non Calculator

A plant has four complete sets of chromosomes derived from one ancestral species after whole-genome duplication. What is this plant?

A.

An analogous structure

B.

An autopolyploid

C.

A sterile interspecific hybrid only

D.

An allopolyploid

Question 16
SL • Paper 2
Medium
Calculator
1.

State one type of molecular sequence used as evidence for evolution.

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2.

Explain why fewer differences in the same gene sequence between two species support a closer evolutionary relationship.

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Question 17
SL • Paper 2
Medium
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A farmer breeds only wheat plants with the largest grains for several generations.

1.

State the type of selection involved.

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2.

Outline how this provides evidence that evolution can occur rapidly.

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Question 18
SL • Paper 2
Medium
Calculator

The forelimbs of humans, bats and whales contain the same major bones in the same relative positions.

Simple labelled comparison of three vertebrate forelimbs showing the same pentadactyl limb bones in corresponding relative positions but with different external shapes.
1.

Name this type of structure.

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2.

Describe how this is evidence for evolution.

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Question 19
SL • Paper 2
Medium
Calculator
1.

Define analogous structures.

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2.

Distinguish analogous structures from homologous structures.

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3.

Give one example of analogous structures.

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Question 20
HL • Paper 2
Medium
Calculator
1.

Define allopatric speciation.

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2.

Define sympatric speciation.

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3.

Compare the requirements for allopatric and sympatric speciation.

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Question 21
HL • Paper 2
Medium
Calculator
1.

State one example of behavioural isolation.

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2.

Distinguish behavioural isolation from temporal isolation.

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Question 22
SL • Paper 1B
Medium
Non Calculator

The table shows the percentage similarity of the cytochrome c amino acid sequence between humans and four other vertebrates.

Vertebrate speciesSimilarity to human / %
Chimpanzee100
Rhesus macaque99
Mouse91
Chicken86
1.

Identify the species most closely related to humans according to the table.

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2.

Describe the relationship between sequence similarity and inferred evolutionary relationship.

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3.

Explain why amino acid sequence data can provide evidence for common ancestry.

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Question 23
SL • Paper 1B
Medium
Non Calculator

A population of domesticated hens was selectively bred for high egg production over several generations. The graph shows mean eggs laid per female per year.

Mean egg production in selectively bred hens, with a target line and standard-error bounds.
1.

State the overall trend shown.

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2.

Estimate the generation in which mean egg production first exceeds the target line shown on the graph.

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3.

Explain how selective breeding could cause the trend.

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4.

Suggest one limitation of using this evidence alone to predict evolution in wild populations.

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Question 24
SL • Paper 1A
Medium
Non Calculator

Evolution by natural selection is described as a scientific theory. What does this mean in biology?

A.

It is a guess that has not been tested experimentally.

B.

It applies only to fossils and not to living populations.

C.

It has been formally proved true by checking all organisms.

D.

It is a broad explanation supported by many observations and successful predictions.

Question 25
HL • Paper 1A
Medium
Non Calculator

Hybridization between two related Persicaria species is followed by chromosome doubling. Why can this cause abrupt speciation?

A.

The new species must occupy the same niche as both parents.

B.

The hybrid immediately loses all chromosomes from both parent species.

C.

The new polyploid may be fertile with similar polyploids but reproductively isolated from parent species.

D.

The parent species become extinct as soon as the hybrid forms.

Question 26
HL • Paper 1A
Medium
Non Calculator

Closely related fish species now live in the same lake. DNA evidence shows they diverged before the lake formed and later colonized it. What conclusion is best supported?

A.

Their present sympatry does not by itself prove sympatric speciation.

B.

They must have speciated sympatrically because they now share a lake.

C.

They cannot be separate species if they live in the same lake.

D.

They must be autopolyploids because DNA differs between them.

Question 27
SL • Paper 2
Medium
Calculator

The Congo River separates bonobos (Pan paniscus) and common chimpanzees (Pan troglodytes).

Map-style diagram showing the Congo River separating the ranges of bonobos and common chimpanzees, with labels for north and south populations and different ecological conditions on each side.
1.

State the type of reproductive isolation caused by the river.

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2.

Explain how this separation could have contributed to speciation.

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Question 28
SL • Paper 2
Medium
Calculator
1.

State why evolution by natural selection is called a theory.

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2.

Outline why evolution by natural selection is regarded as a pragmatic truth in biology.

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Question 29
HL • Paper 2
Medium
Calculator

Galápagos finches include closely related species with different beak shapes and diets.

IB-style diagram of several related finch species branching from one ancestor, with differently shaped beaks labelled for different diets or feeding niches.
1.

Name the evolutionary pattern represented.

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2.

Explain how this pattern can increase biodiversity.

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Question 30
HL • Paper 2
Medium
Calculator
1.

State one prezygotic barrier that can prevent hybridization in animals.

[1]
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2.

Explain how this barrier helps maintain species boundaries.

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Question 31
HL • Paper 2
Medium
Calculator

A horse and a donkey can produce a mule, but mules are usually sterile.

1.

Identify whether mule sterility is a prezygotic or postzygotic barrier.

[1]
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2.

Explain why mule sterility prevents mixing of alleles between horses and donkeys.

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Question 32
HL • Paper 2
Medium
Calculator
1.

Define polyploidy.

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2.

Distinguish autopolyploidy from allopolyploidy.

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Question 33
SL • Paper 1B
Medium
Non Calculator

The diagram compares the forelimb bones of four vertebrates.

Comparative diagram of four vertebrate forelimbs with corresponding bones labelled by colour or name, showing a conserved pentadactyl pattern modified for different functions.
1.

Identify one bone that is present in all four limbs.

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2.

State the term used for structures with this pattern of similarity.

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3.

Explain how the diagram supports common ancestry despite different limb functions.

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Question 34
SL • Paper 1B
Medium
Non Calculator

Two populations of a river fish became separated when a waterfall formed. The graph shows allele frequency at one locus in the upstream and downstream populations over time.

Line graph showing frequency of allele A over time in upstream and downstream fish populations after waterfall formation.
1.

Compare the allele frequencies in the two populations at the start of the study.

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2.

Describe how the allele frequencies change after separation.

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3.

Explain how reproductive isolation and differential selection could lead to speciation in this example.

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Question 35
HL • Paper 1B
Medium
Non Calculator

The figure shows three populations of crickets. Populations X and Y are separated by a mountain range. Populations Y and Z live in the same valley, but males sing at different times of night.

Map-style diagram with cricket populations X, Y and Z, a mountain barrier separating X from Y, and an inset showing different nightly calling times for Y and Z in the same valley.
1.

Identify the isolation mechanism between X and Y.

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2.

Identify the isolation mechanism between Y and Z.

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3.

Compare how allopatric and sympatric speciation could occur in these crickets.

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Question 36
HL • Paper 1B
Medium
Non Calculator

The graph shows beak depth and main food source for several related finch species on an island group.

Mean beak depth by main food source for related island finches.
1.

Identify the species most adapted for feeding on large hard seeds.

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2.

Describe the relationship between beak depth and main food source.

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3.

Explain how the pattern shown is evidence of adaptive radiation.

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Question 37
HL • Paper 2
Medium
Calculator

In knotweeds and smartweeds (Persicaria), new species can form after hybridization followed by chromosome doubling.

Flowchart showing two related *Persicaria* parent species forming a sterile hybrid, followed by chromosome doubling to a fertile polyploid lineage reproductively isolated from both parents.
1.

Name this form of polyploid speciation.

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2.

Explain why chromosome doubling can restore fertility in the hybrid.

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Question 38
HL • Paper 2
Medium
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Two sister species occur on the same island. Researchers suggest they formed by sympatric speciation.

1.

State one observation that would support sympatric rather than allopatric speciation.

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2.

Explain why the current presence of both species on the island is not sufficient evidence.

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Question 39
SL • Paper 1B
Hard
Non Calculator

The table compares wings of three animal groups.

Animal groupMain functionStructural originBones presentDevelopmental originMajor support tissue
BirdPowered flightModified forelimbYesVertebrate limb budBone and feathers
BatPowered flightModified forelimbYesVertebrate limb budBone and skin membrane
InsectPowered flightBody wall outgrowthNoInsect wing padChitin cuticle
1.

Identify the pair of wings that are most likely analogous.

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2.

Give one item of evidence from the table supporting your answer to (a).

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3.

Explain how convergent evolution could produce the similarity.

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4.

Suggest one additional type of evidence that would help distinguish analogy from homology.

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Question 40
HL • Paper 1B
Hard
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Researchers measured female response to male courtship songs in two closely related grasshopper species. The bar chart shows the percentage of females that approached different song types.

Percentage of females approaching three male song types in two grasshopper species.
1.

Identify the song type producing the highest response in females of species A.

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2.

Compare the responses of species A and species B females to hybrid songs.

[1]
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3.

Explain how courtship behaviour can act as a barrier to hybridization.

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4.

Suggest why a weak response to hybrid songs could maintain separate gene pools.

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Question 41
HL • Paper 1B
Hard
Non Calculator

The table shows chromosome numbers and fertility of crosses among two diploid Persicaria species, their hybrid, and a chromosome-doubled derivative.

IndividualChromosome set originTotal chromosomes / 2nPollen fertility / %Crosses with parents / seeds flower^-1Crosses within same type / seeds flower^-1
P. species AAA22943134
P. species BBB20912830
F1 hybridAB2130.40.2
Doubled derivativeAABB42821.126
1.

Identify the individual most likely to be an allopolyploid.

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2.

State the evidence from the table that the initial hybrid is reproductively isolated.

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3.

Explain why chromosome doubling can increase fertility.

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4.

Suggest why the chromosome-doubled derivative may be considered a new species.

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Question 42
SL • Paper 2
Hard
Calculator
1.

Outline the difference between Darwinian evolution and Lamarckism.

[1]
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2.

Discuss why evolution by natural selection is regarded as a scientific theory with strong evidential support.

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Question 43
SL • Paper 2
Hard
Calculator
Diagram comparing a human arm, bat wing, whale flipper and insect wing, with vertebrate limb bones labelled for the first three and structural differences in the insect wing indicated.
1.

Describe the pentadactyl limb as an example of a homologous structure.

[1]
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2.

Compare and contrast homologous and analogous structures as evidence for evolution.

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Question 44
SL • Paper 2
Hard
Calculator
1.

Outline how selective breeding demonstrates evolutionary change.

[1]
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2.

Evaluate the use of molecular sequence data and selective breeding as evidence for evolution.

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Question 45
HL • Paper 1B
Hard
Non Calculator

A phylogeny and distribution map are shown for four related snail species. Species 1 and 2 live on the same coast today. The phylogeny estimates divergence times, and the map shows historical coastlines and barriers.

NodeDaughter lineagesSplit time / MaPresent-day rangeCoastal setting at split
ASpecies 1 \Species 20.52Both on east coast; 45 km overlapSand barrier divided north and south coves
BSpecies 3 \Species 41.10Species 3 west island; Species 4 south capeDeep channel separated island and cape
CNode A \Node B2.40All within same coastal regionLow sea level exposed discontinuous rocky shore
1.

Identify whether species 1 and 2 are sister species.

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2.

State one item of evidence suggesting they may have diverged allopatrically.

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3.

Explain why present-day sympatry is not enough to infer sympatric speciation.

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4.

Suggest one additional data type that would strengthen the conclusion about mode of speciation.

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Question 46
SL • Paper 2
Hard
Calculator
1.

Outline speciation by splitting of a pre-existing species.

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2.

Explain the roles of reproductive isolation and differential selection in speciation, using the Congo River example.

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Question 47
HL • Paper 2
Hard
Calculator
1.

Distinguish geographic, behavioural and temporal isolation.

[1]
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2.

Compare and contrast allopatric and sympatric speciation.

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Question 48
HL • Paper 2
Hard
Calculator
Branching diagram of a founder lineage radiating into several related species occupying distinct ecological niches, with labels for resources and habitats.
1.

Define adaptive radiation and ecological niche.

[1]
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2.

Explain how adaptive radiation can increase biodiversity and allow coexistence of related species.

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Question 49
HL • Paper 2
Hard
Calculator
1.

Distinguish prezygotic and postzygotic reproductive barriers.

[1]
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2.

Discuss how barriers to hybridization and hybrid sterility prevent mixing of alleles between species.

[1]
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Question 50
HL • Paper 2
Hard
Calculator
Stepwise diagram of allopolyploid speciation in *Persicaria*: two diploid parent species, sterile hybrid, whole-genome duplication, fertile polyploid lineage, and reproductive isolation from parent species.
1.

Distinguish autopolyploidy from allopolyploidy.

[1]
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2.

Explain how hybridization and polyploidy can cause abrupt speciation in plants, using Persicaria as an example.

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A3.2 Classification and cladistics