The student does not clearly state the independent and dependent variables in the research question, as it lacks specific details about measuring glucose concentration in degrees Brix (°Bx). However, the student effectively describes the method of analysis by specifying how the effect of milk pH on lactose hydrolysis is assessed through refractometry. The background theory is well articulated, demonstrating a solid understanding of the biochemical principles involved. The methodology is sufficiently detailed, explaining the choice of techniques, sampling, and controlled variables, while also addressing safety, ethical, and environmental issues associated with the investigation. Overall, the student presents a coherent and well-structured investigation despite the initial lack of clarity in the research question.