The student clearly states the independent variable as the concentration of Nigella sativa extract and the dependent variable as the percentage of amylase inhibition. The research question includes a detailed description of the analysis method, utilizing color change in the iodine test and absorbance readings from a colorimeter. A thorough background theory is provided, explaining the interaction of Nigella sativa's phytochemicals with enzymes, and the choice of techniques for measuring variables is well justified. The student effectively identifies controlled variables, safety, ethical, and environmental issues, and describes the methodology in sufficient detail for reproducibility, ensuring clarity and relevance throughout the investigation.
