The student clearly stated the independent variable as the type of plant extract and the dependent variable as the growth of Escherichia coli, with appropriate methods of analysis described. The choice of techniques for measuring these variables was well explained, particularly the use of UV-visible spectrophotometry. However, the student did not adequately address certain aspects, such as the background theory relevant to the research question, the rationale behind the number of trials conducted, and the specification of controlled variables. Additionally, safety and environmental issues associated with the investigation were not sufficiently addressed, although ethical considerations were mentioned. Overall, while the methodology was mostly clear and coherent, there were critical areas that required improvement for a more comprehensive understanding.