The student clearly states the independent and dependent variables in the research question, identifying various plant-based protein sources as the independent variable and BCAA concentration as the dependent variable. The method of analysis, which involves thin layer chromatography (TLC), is also well described. Background theory relevant to the research question is provided, including the significance of BCAAs for vegan athletes and the rationale for choosing specific plant sources. The student identifies controlled variables and explains how they were managed, while also addressing safety, ethical, and environmental issues associated with the investigation. However, there is a lack of clarity regarding the sampling technique and the rationale for the number of trials conducted, which should be elaborated to enhance the methodology's robustness. Overall, the methodology is described sufficiently for reproducibility, with no irrelevant information present.
